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KMID : 0624620100430040257
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BMB Reports
2010 Volume.43 No. 4 p.257 ~ p.262
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Influence of the lung mechanical ventilation with injurious parameters on 7-ketocholesterol synthesis in Sus Scrofa
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Klimenko Oxana V.
Vobruba Vaclav
Martasek Pavel
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Abstract
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The aim of work was to investigate changes of 7-ketocholesterol synthesis in alveolar macrophages in the dynamic of lung mechanical ventilation with injurious parameters. The goal of in vitro part of work was to observe influence of 7-ketocholesterol on iNOS and MIP1 β production in bronchoalveolar lavage fluid (BALF) cells. We used 17 healthy domestic pigs randomly assigned into two treatment groups: group I with mechanical ventilation with physiological parameters; group II underwent injurious ventilation with high volume tidal (VT) and low positive end expiratory pressure (PEEP). Сells were analyzed for CYP27A1 protein and gene expression levels, 7-ketocholesterol production. In alveolar macrophages of group II, we obtained increase of production of CYP27A1 protein and 7-ketocholesterol, as well as the expression of the CYP27A1 gene at the 2nd hour of ventilation. In the in vitro experiments we show dose-dependent increase of MIP1 β and decrease of CYP27A1, iNOS protein production after 7-ketocholesterol treatment.
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KEYWORD
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BALF, CYP27A1, iNOS, MIP1 β, 7-ketcholesterol
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